[HTML][HTML] The Plasmodium falciparum circumsporozoite protein produced in Lactococcus lactis is pure and stable
SK Singh, J Plieskatt, BK Chourasia, V Singh… - Journal of Biological …, 2020 - ASBMB
The Plasmodium falciparum circumsporozoite protein (PfCSP) is a sporozoite surface
protein whose role in sporozoite motility and cell invasion has made it the leading candidate
for a pre-erythrocytic malaria vaccine. However, production of high yields of soluble
recombinant PfCSP, including its extensive NANP and NVDP repeats, has proven
problematic. Here, we report on the development and characterization of a secreted,
soluble, and stable full-length PfCSP (containing 4 NVDP and 38 NANP repeats) produced …
protein whose role in sporozoite motility and cell invasion has made it the leading candidate
for a pre-erythrocytic malaria vaccine. However, production of high yields of soluble
recombinant PfCSP, including its extensive NANP and NVDP repeats, has proven
problematic. Here, we report on the development and characterization of a secreted,
soluble, and stable full-length PfCSP (containing 4 NVDP and 38 NANP repeats) produced …