The Effects of radiation on the safety and protective efficacy of an attenuated Plasmodium yoelii sporozoite malaria vaccine
R Chattopadhyay, S Conteh, ML Li, ER James… - Vaccine, 2009 - Elsevier
R Chattopadhyay, S Conteh, ML Li, ER James, JE Epstein, SL Hoffman
Vaccine, 2009•ElsevierWe are developing a radiation attenuated Plasmodium falciparum sporozoite (PfSPZ)
malaria vaccine. An important step was to determine the minimum dose of irradiation
required to adequately attenuate each sporozoite. This was studied in the Plasmodium yoelii
rodent model system. Exposure to 100Gy completely attenuated P. yoelii sporozoites
(PySPZ). Next we demonstrated that immunization of mice intravenously with 3 doses of 750
PySPZ that had received 200Gy, double the radiation dose required for attenuation, resulted …
malaria vaccine. An important step was to determine the minimum dose of irradiation
required to adequately attenuate each sporozoite. This was studied in the Plasmodium yoelii
rodent model system. Exposure to 100Gy completely attenuated P. yoelii sporozoites
(PySPZ). Next we demonstrated that immunization of mice intravenously with 3 doses of 750
PySPZ that had received 200Gy, double the radiation dose required for attenuation, resulted …
We are developing a radiation attenuated Plasmodium falciparum sporozoite (PfSPZ) malaria vaccine. An important step was to determine the minimum dose of irradiation required to adequately attenuate each sporozoite. This was studied in the Plasmodium yoelii rodent model system. Exposure to 100Gy completely attenuated P. yoelii sporozoites (PySPZ). Next we demonstrated that immunization of mice intravenously with 3 doses of 750 PySPZ that had received 200Gy, double the radiation dose required for attenuation, resulted in 100% protection. These results support the contention that a radiation attenuated sporozoite vaccine for malaria will be safe and effective at a range of radiation doses.
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