The ISG15 conjugation system
LA Durfee, JM Huibregtse - Ubiquitin Family Modifiers and the Proteasome …, 2012 - Springer
LA Durfee, JM Huibregtse
Ubiquitin Family Modifiers and the Proteasome: Reviews and Protocols, 2012•SpringerISG15 is a ubiquitin-like modifier that is expressed in response to type 1 interferon signaling
(IFN-α/β) and plays a role in antiviral responses. The core E1, E2, and E3 enzymes for
ISG15 are Ube1L, UbcH8, and Herc5, respectively, and these are all also induced at the
transcriptional level by IFN-α/β. We recently showed that Herc5 associates with polysomes
and modifies target proteins in a cotranslational manner. Here, we describe the expression
of the core conjugating enzymes in human cells, the detection of ISG15 conjugates, and the …
(IFN-α/β) and plays a role in antiviral responses. The core E1, E2, and E3 enzymes for
ISG15 are Ube1L, UbcH8, and Herc5, respectively, and these are all also induced at the
transcriptional level by IFN-α/β. We recently showed that Herc5 associates with polysomes
and modifies target proteins in a cotranslational manner. Here, we describe the expression
of the core conjugating enzymes in human cells, the detection of ISG15 conjugates, and the …
Abstract
ISG15 is a ubiquitin-like modifier that is expressed in response to type 1 interferon signaling (IFN-α/β) and plays a role in antiviral responses. The core E1, E2, and E3 enzymes for ISG15 are Ube1L, UbcH8, and Herc5, respectively, and these are all also induced at the transcriptional level by IFN-α/β. We recently showed that Herc5 associates with polysomes and modifies target proteins in a cotranslational manner. Here, we describe the expression of the core conjugating enzymes in human cells, the detection of ISG15 conjugates, and the methods for fractionation of Herc5 with polysomes.
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