[HTML][HTML] Rpn7 Is required for the structural integrity of the 26 S proteasome of Saccharomyces cerevisiae
E Isono, Y Saeki, H Yokosawa, A Toh-e - Journal of Biological Chemistry, 2004 - ASBMB
Rpn7 is one of the lid subunits of the 26 S proteasome regulatory particle. The RPN7 gene is
known to be essential, but its function remains to be elucidated. To explore the function of
Rpn7, we isolated and characterized temperature-sensitive rpn7 mutants. All of the rpn7
mutants obtained accumulated poly-ubiquitinated proteins when grown at the restrictive
temperature. The N-end rule substrate (Ub-Arg-β-galactosidase), the UFD pathway
substrate (Ub-Pro-β-galactosidase), and cell cycle regulators (Pds1 and Clb2) were found to …
known to be essential, but its function remains to be elucidated. To explore the function of
Rpn7, we isolated and characterized temperature-sensitive rpn7 mutants. All of the rpn7
mutants obtained accumulated poly-ubiquitinated proteins when grown at the restrictive
temperature. The N-end rule substrate (Ub-Arg-β-galactosidase), the UFD pathway
substrate (Ub-Pro-β-galactosidase), and cell cycle regulators (Pds1 and Clb2) were found to …