Recombinant human antibody single chain variable fragments reactive with Candida albicans surface antigens
CG Haidaris, J Malone, LA Sherrill, JM Bliss… - Journal of …, 2001 - Elsevier
A combinatorial phage display library expressing human immunoglobulin heavy and light
chain variable regions was used to identify phage clones capable of binding to the surface
of Candida albicans blastoconidia. Single chain antibody variable fragments (scFv) derived
from three clones detected C. albicans antigens by indirect immunofluorescence assay
(IFA), enzyme-linked immunosorbent assay (ELISA), and Western blotting. The antigens
detected were conserved among different strains of C. albicans and several other Candida …
chain variable regions was used to identify phage clones capable of binding to the surface
of Candida albicans blastoconidia. Single chain antibody variable fragments (scFv) derived
from three clones detected C. albicans antigens by indirect immunofluorescence assay
(IFA), enzyme-linked immunosorbent assay (ELISA), and Western blotting. The antigens
detected were conserved among different strains of C. albicans and several other Candida …