Glutamine has recently emerged as a key substrate to support cancer cell proliferation, and
the quantification of its metabolic flux is essential to understand the mechanisms by which
this amino acid participates in the metabolic rewiring that sustains the survival and growth of
neoplastic cells. Glutamine metabolism involves two major routes, glutaminolysis and
reductive carboxylation, both of which begin with the deamination of glutamine to glutamate
and the conversion of glutamate into α-ketoglutarate. In glutaminolysis, α-ketoglutarate is …