Genome-scale DNA methylation mapping of clinical samples at single-nucleotide resolution
Nature methods, 2010•nature.com
Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide
resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite
sequencing for genome-scale analysis of clinical samples: here we outline how restriction
digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a
statistical method for assessing significance of altered DNA methylation patterns. Thirty
nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well …
resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite
sequencing for genome-scale analysis of clinical samples: here we outline how restriction
digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a
statistical method for assessing significance of altered DNA methylation patterns. Thirty
nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well …
Abstract
Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples.
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