[47] Construction of encapsidated (gutted) adenovirus minichromosomes and their application to rescue of photoreceptor degeneration
R Kumar-Singh, CK Yamashita, K Tran… - Methods in enzymology, 2000 - Elsevier
Publisher Summary This chapter includes all the protocols required to construct and test
EAMs with any transgene of interest. A discussion of the main features of the different types
of EAMs and of the modified protocols, developed for analysis of EAM-mediated expression
of transgenes in the retina has been provided. The construction of EAMs depends on two
seminal observations regarding the nature of the origin of replication and encapsidation
signals of adenovirus (Ad). EAMs were constructed to address the issue of the strong host …
EAMs with any transgene of interest. A discussion of the main features of the different types
of EAMs and of the modified protocols, developed for analysis of EAM-mediated expression
of transgenes in the retina has been provided. The construction of EAMs depends on two
seminal observations regarding the nature of the origin of replication and encapsidation
signals of adenovirus (Ad). EAMs were constructed to address the issue of the strong host …
