The identification of Trypanosoma brucei subspecies using repetitive DNA sequences
G Hide, P Cattand, D LeRay, JD Barry, A Tait - Molecular and Biochemical …, 1990 - Elsevier
We describe the use of repetitive DNA probes to characterise the relationships between
different stocks of African trypanosomes representing the subspecies of Trypanosoma
brucei. Probes derived from the ribosomal RNA genes (coding region and non-transcribed
spacer) and another repetitive DNA sequence were used to characterise trypanosome
stocks by Southern blotting. Numerical taxonomy methods applied to the resulting restriction
enzyme patterns were used to derive a dendrogram depicting the relationships between the …
different stocks of African trypanosomes representing the subspecies of Trypanosoma
brucei. Probes derived from the ribosomal RNA genes (coding region and non-transcribed
spacer) and another repetitive DNA sequence were used to characterise trypanosome
stocks by Southern blotting. Numerical taxonomy methods applied to the resulting restriction
enzyme patterns were used to derive a dendrogram depicting the relationships between the …
