Production of anti-peptide antibodies against trypanopain-Tb from Trypanosoma brucei brucei: effects of antibodies on enzyme activity against Z-Phe-Arg-AMC
L Troeberg, RN Pike, JD Lonsdale-Eccles… - …, 1997 - Elsevier
Anti-peptide antibodies were produced against the cysteine proteinase trypanopain-Tb from
Trypanosoma brucei brucei and the effects of these antibodies on enzyme activity against
carboxybenzoyl (Z)-Phe-Arg-aminomethylcoumarin (AMC) investigated. A peptide was
synthesised corresponding to a region of the trypanopain-Tb active site around the active
site histidine so that the resulting anti-peptide antibodies specifically targeted the active site
of the enzyme. Such antibodies were considered more likely to modulate enzyme activity …
Trypanosoma brucei brucei and the effects of these antibodies on enzyme activity against
carboxybenzoyl (Z)-Phe-Arg-aminomethylcoumarin (AMC) investigated. A peptide was
synthesised corresponding to a region of the trypanopain-Tb active site around the active
site histidine so that the resulting anti-peptide antibodies specifically targeted the active site
of the enzyme. Such antibodies were considered more likely to modulate enzyme activity …
